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Filtered Search Results
Thermo Scientific™ KpnI (10 U/μL)
The KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Asp718I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Kpn I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Unique Buffer (10X Buffer KpnI) |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest VspI
Cut at AT^TAAT sites with Thermo Scientific™ FastDigest™ AseI (VspI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PshBI).
Thermo Scientific™ HpaII (10 U/μL)
The HpaII restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Hpa II |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ MluI (10 U/μL)
The MluI restriction enzyme recognizes A^CGCGT sites and cuts best at 37°C in R buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Mlu I |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer R |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest BclI
Cut at T^GATCA sites with Thermo Scientific™ FastDigest™ BclI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FbaI, Ksp22I).
Thermo Scientific™ FastDigest AluI
Cut at AG^CT sites with Thermo Scientific™ FastDigest™ AluI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AluBI).
Thermo Scientific™ FastDigest PdmI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)
Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).
Thermo Scientific™ Eco130I (StyI) (10 U/μL)
Cut at C^CWWGG sites with Eco130I (StyI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: BssT1I, EcoT14I, ErhI, StyI).
Thermo Scientific™ SdaI (SbfI) (10 U/μL)
The SdaI (SbfI) restriction enzyme recognizes CCTGCA^GG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: SbfI, Sse8387I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | SdaI (SbfI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Unique Buffer (10X Buffer SdaI (SbfI)) |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest Alw44I
Cut at G^TGCAC sites with Thermo Scientific™ FastDigest™ ApaLI (Alw44I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: VneI).
Thermo Scientific™ NmuCI (Tsp45I) (10 U/μL)
Cut at ^GTSAC sites with NmuCI (Tsp45I) restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: Tsp45I).
Thermo Scientific™ XmaJI (AvrII) (10 U/μL)
Cut at C^CTAGG sites with XmaJI (AvrII) restriction enzyme, which functions best at 37°C in Tango buffer (Isoschizomers: AspA2I, AvrII, BlnI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | XmaJI (AvrII) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ SsiI (AciI) (10 U/μL)
Cut at CCGC(-3/-1)^ sites with SsiI (AciI) restriction enzyme, which functions best at 37°C in O buffer (Isoschizomers: AciI, BspACI).
Thermo Scientific™ SgsI (AscI) (10 U/μL)
The SgsI (AscI) restriction enzyme recognizes GG^CGCGCC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AscI, PalAI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | SgsI (AscI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |